✦ LIBER ✦

Rapid isolation of bacterial proteinases by column chromatography on sawdust

✍ Scribed by Dr. I. Šafařík

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 168 KB
Volume: 25
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620251023

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✦ Synopsis

Proteolytic enzymes produced by some strains of Bacillus sp. were isolated by column chromatography on sawdust. Ballast proteins were eluted with water while the adsorbed proteinases were eluted with 1 M sodium chloride solution. The specific activities of proteinases increased 54-to 70-fold after chromatography.

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