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Rapid Isolation of Active Mitochondria from Plant Tissue

✍ Scribed by PALMER, J. M.


Book ID
109659970
Publisher
Nature Publishing Group
Year
1967
Tongue
English
Weight
143 KB
Volume
216
Category
Article
ISSN
0028-0836

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A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chr