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Rapid immunofluorescent detection of a viral infection in tissue culture

✍ Scribed by H.H. Weetall; A.A. Luderer; H.C. Orr; P.G. Probst

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
209 KB
Volume
1
Category
Article
ISSN
0165-2478

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Ohio HeLa cells in multichamber slides were inoculated with nasal samples from patients presenting with common cold symptoms and incubated at 33Β°C with gentle shaking for 48 hours. The cultures were fixed with cold acetone, and viral antigens were detected by immunofluorescence using an antirhinovir