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Rapid identification of Triticeae genotypes from single seeds using the polymerase chain reaction

✍ Scribed by C. Benito; A. M. Figueiras; C. Zaragoza; F. J. Gallego; A. Peña

Publisher
Springer
Year
1993
Tongue
English
Weight
406 KB
Volume
21
Category
Article
ISSN
0167-4412

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✦ Synopsis

An easy and quick protocol has been developed for DNA analysis via PCR. Single cereal endosperm or small leaf pieces can be separately processed in several PCR reactions. The resultant PCR patterns are equivalents to those obtained with standard DNA extraction protocols using either specific or random primers. Intra-and inter-specific variability can be detected. This method allows the analysis of a large number of individuals in early stages prior to the plant sowing.

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