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Rapid identification of N-glycosylation sites in a glycoprotein by collision-induced dissociation mass spectrometry

โœ Scribed by Javier Gonzalez; Toshifumi Takao; Vladimir Besada; Rolando Rodriguez; Gabriel Padron; Yasutsugu Shimonishi


Book ID
105274023
Publisher
Springer
Year
1992
Tongue
English
Weight
243 KB
Volume
11
Category
Article
ISSN
1573-4943

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A method for determination of N-glycosyl
โœ Javier Gonzalez; Toshifumi Takao; Hideaki Hori; Vladimir Besada; Rolando Rodrigu ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 1006 KB

Previously, a combined use of fast atom bombardment (FAB) mass spectrometry and peptide N-glycosidase F, an enzyme that cleaves the beta-aspartylglycosylamine linkage of Asn-linked carbohydrates, was successfully applied to identification of N-glycosylation sites in a glycoprotein with the known or