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Rapid identification of cytochrome P450cam variants by in vivo screening of active site libraries

โœ Scribed by Robert E. Speight; Fred E. Hancock; Chris Winkel; Hanamanthsa S. Bevinakatti; Manish Sarkar; Sabine L. Flitsch; Nicholas J. Turner


Book ID
108283809
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
313 KB
Volume
15
Category
Article
ISSN
0957-4166

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Hydration of protein cavities influences protein stability, dynamics, and function. Protein active sites usually contain water molecules that, upon ligand binding, are either displaced into bulk solvent or retained to mediate protein-ligand interactions. The contribution of water molecules to ligand