✦ LIBER ✦

Rapid, high-sensitivity imaging of radiolabeled gels with microchannel plate detectors

✍ Scribed by John E. Lees; Paul G. Richards

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 125 KB
Volume: 20
Category: Article
ISSN: 0173-0835
DOI: 10.1002/(sici)1522-2683(19990701)20:10<2139::aid-elps2139>3.0.co;2-r

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✦ Synopsis

A michrochannel plate detector has been used to image tritium-labeled protein on oneand two-dimensional electrophoresis gels. The good spatial resolution (70 microns) and high sensitivity (6.0 dpm/mm 2 ) of the imaging system allows detection of low levels (femto moles) of labelled proteins. We are currently using the detector for identification of new targets involved in organophosphate neurotoxicity.

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