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Rapid high-pressure liquid chromatographic determination of methotrexate and its metabolites 7-hydroxymethotrexate and 2,4-diamino-N10-methylpteroic acid in biological fluids

✍ Scribed by H. Breithaupt; E. Küenzlen; G. Goebel

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
828 KB
Volume
121
Category
Article
ISSN
0003-2697

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✍ F. Bressolle; J. Bres; A. Moulin 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 496 KB

A high-performance liquid chromatographic method with spectrofluorometric detection has been developed for the analysis of a new substituted benzamide and its metabolite in body fluids. The detection was performed at 270 nm for excitation, and 370 nm for emission. The proposed technique is selective