The effect of pH on the elongation rate of 2-mm-long subapical segments from corn roots (Zea mays L.) was investigated under aerobic (O2) and anaerobic (N2) conditions using a specially designed apparatus. The maximum elongation rate was obtained with a citrate-phosphate buffered solution of pH 3.4
Rapid-growth responses of corn root segments: Effect of citrate-phosphate buffer on elongation
โ Scribed by Kathryn L. Edwards; Tom K. Scott
- Publisher
- Springer-Verlag
- Year
- 1976
- Tongue
- English
- Weight
- 465 KB
- Volume
- 129
- Category
- Article
- ISSN
- 0032-0935
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โฆ Synopsis
The effects of citrate-phosphate buffer on the elongation rate of 2 mm root segments of Zea mays L. was investigated under 02 and N2 conditions by comparing with the effects in non-buffered solution. The buffer at pH 7.0 has an inhibiting effect on the elongation rate of root segments. At pH 4.0 the buffer has a promoting effect on the elongation rate twice that of the H-ion concentration. Nitrogen experiments indicate that growth in neutral buffer is O2 dependent. On the other hand the acid-growth response of root segments in citrate-phosphate buffer appears to have two components, 1) a H-ion component which is partially inhibited by N2 and fluoride and 2) a citrate component which is inhibited in part by N 2 but not by fluoride.
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