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Rapid genetic mapping in Neurospora crassa

✍ Scribed by Yuan Jin; Sabrina Allan; Lauren Baber; Eric K. Bhattarai; Teresa M. Lamb; Wayne K. Versaw


Book ID
116481803
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
415 KB
Volume
44
Category
Article
ISSN
1087-1845

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Liposome-mediated genetic transformation
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Transformation of Neurospora crassa spheroplasts is reported for three different genes, using uncloned Neurospora DNA, both naked and encapsulated in synthetic phosphatidylserine liposomes. Whereas transformation by naked DNA is DNase-sensitive, that by liposomes is not. Per unit of transforming DNA