Rapid fluorometric assay for mitochondrial proton adenosine triphosphatase activity for assessment of viability of liver graft tissue

✍ Scribed by Kazuhiko Seya; Nobuhiro Ohkohchi; Nobuyuki Watanabe; Hiroshi Shibuya; Yoshio Taguchi; Shozo Mori

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 527 KB
• Volume: 8
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860080613

✦ Synopsis

We developed an improved determination method of mitochondrial proton adenosine triphosphatase (ATPase) activity in the liver. The activity was measured fluorometrically with a 3,3'-dipropylthiodicarbocyanine iodide (diS-C3( )), which is excited at 625 nm and emits fluorescence at 670 nm.This dye transmits the electric potential across the inner mitochondrial membrane. The fluorescence intensity of diS-Cs(5) with mitochondria (100