Rapid evaluation of peat-base legume inoculant using immunomagnetic beads for cell retrieval and fluorescent nucleic acid probes for viability analysis
✍ Scribed by P. E. Olsen; W. A. Rice
- Book ID
- 104616531
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 330 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0032-079X
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✦ Synopsis
Problems associated with traditional methods of evaluating legume inoculants using the most-probable-number plant nodule grow-out test include technician time, expense, growth chamber space, and the length of time (30 days) required to conduct the tests. Through the use of specific monoclonal antibodies, a biotin-labelled intermediate antibody, and streptavidin-labelled magnetic micro-beads, we were able to rapidly remove and purify rhizobial cells from peat inoculants used as quality control samples in the Canadian Legume Inoculant and Pre-Inoculated Seed Testing Program. Viability of the recovered cells was evaluated with a commercial kit employing differential fluorescent nucleic acid probes allowing viability to be rapidly determined via visual examination with a fluorescence microscope. Total elapsed time for complete evaluation of an inoculant was about 90 minutes.