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Rapid Estimation of Relative Protein—Ligand Binding Affinities Using a High-Throughput Version of MM-PBSA.

✍ Scribed by Scott P. Brown; Steven W. Muchmore


Book ID
102005185
Publisher
John Wiley and Sons
Year
2007
Weight
11 KB
Volume
38
Category
Article
ISSN
0931-7597

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✦ Synopsis


Abstract

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Low-Affinity Binding Determined by Titra
✍ Yan-Ling Zhang; Zhong-Yin Zhang 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 187 KB

A competition-based method is used for the determination of the thermodynamic parameters for a low-affinity ligand binding reaction by isothermal titration calorimetry. This method is based on the coupling of a high-affinity ligand to the binding of the low-affinity ligand. Results are presented for