Rapid enhanced chemiluminescent enzyme immunoassay for ferritin monitored using instant photographic film

✍ Scribed by Angela O'Toole; Larry J. Kricka; Gary H.G. Thorpe; Thomas P. Whitehead

• Book ID: 102983714
• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 522 KB
• Volume: 266
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85042-5

✦ Synopsis

A rapid chemiluminescent enzyme immunometnc assay (EIA) for serum femtin has been developed which combines a halophenol-enhanced chemilummescent assay for the peroxidase label with photographic detection (20000 ASA instant film) of light emission Assays were performed using a microtitre plate as the solid support and a specially designed camera lummometer to measure light emission from individual antibody coated wells The assay operates as a threshold test above a certain serum femtm concentration the film was fully or partially exposed whilst below this level the film remained unexposed The effect of incubation time and temperature were investigated and two rapid mimunoassays were developed In one, a 10-mm incubation with sample and with conjugate detected 1 ng (10 µg 1 -1 ) of ferntm, whilst in the other, 3-mm incubations at ambient temperature detected 40 ng (400 lAg I -') of femtin These assays were reliable, produced a permanent photographic record of the results and the fastest assay was completed in less than 10 min