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Rapid engineering of bacterial artificial chromosomes using oligonucleotides

✍ Scribed by Srividya Swaminathan; Hilary M. Ellis; Laura S. Waters; Daiguan Yu; E-Chiang Lee; Donald L. Court; Shyam K. Sharan

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
288 KB
Volume
29
Category
Article
ISSN
1526-954X

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## Summary A porcine bacterial artificial chromosome (BAC) library consisting of 103 488 clones has been constructed. The average insert size in the BAC vector was calculated to be 133 kb based on the examination of 189 randomly selected clones, indicating that the library contained 4.4 genome equi