Rapid DNA mutation identification and fingerprinting using base excision sequence scanning
โ Scribed by Gregory A. Hawkins; Les M. Hoffman
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 414 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Next-generation sequencing (NGS) technologies can be a boon to human mutation detection given their high throughput: consequently, many genes and samples may be simultaneously studied with high coverage for accurate detection of heterozygotes. In circumstances requiring the intensive study of a few
Direct sequencing analysis is largely used to confirm and characterize mutations previously detected by more rapid tests. We have developed a method-Comparative Sequence Analysis (CSA)that simplifies the analysis of sequencing data facilitating its use as a first screen for mutation detection. Seque
Several approaches are now available for screening populations for known mutations in a given gene. However, for detection of multiple mutations in a population that has not been characterized or for detection of new mutations, the value and efficiency of these screening procedures decreases. Althou
A novel strategy is described for highly sensitive DNA detection and point mutation identification based on the combination of reverse molecular beacon with DNA ligase. A 5'-phosphoryl and 3'-ferrocene terminated DNA sequence is used as detection probe, which may be ligated to capture DNA immobilize