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Rapid determination of phytanic acid in human blood serum by selectivity tuning in packed column SFC

✍ Scribed by Lembke, Peter ;Engelhardt, Heinz

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 259 KB
Volume: 16
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240161205

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✦ Synopsis

Abstract

A new packed column SFC method has been developed for the determination of phytanic acid in human blood serum. Because of the low viscosity and high diffusion coefficient of supercritical carbon dioxide, several columns can be connected in series, significantly improving separation. Series connection of columns which differ in their selectivity enables the adjustment of the total selectivity towards the individual separation problem (‘selectivity tuning’). In this instance it was possible, after transesterification into their ethyl esters, to separate all the fatty acids in human blood serum, including phytanic acid, by coupling three columns (silica, amino‐propyl, and RP8) in series.

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