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Rapid determination of free proline for water-stress studies

✍ Scribed by L. S. Bates; R. P. Waldren; I. D. Teare


Book ID
104614536
Publisher
Springer
Year
1973
Tongue
English
Weight
136 KB
Volume
39
Category
Article
ISSN
0032-079X

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✦ Synopsis


Proline, which increases proportionately faster than other amino acids in plants under water stress, has been suggested as an evaluating parameter for irrigation scheduling and for selecting drought-resistant varieties. The necessity to analyze numerous samples from multiple replications of field grown materials prompted the development of a simple, rapid colorimetric determination of proline. The method detected proline in the 0.1 to 36.0 ~moles/g range of fresh weight leaf material.


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## Abstract The sample of superphosphate is extracted with water, and the solution titrated with carbonate‐free sodium hydroxide in two stages with appropriate indicators. The first stage gives the free acid, and the second stage an approximate result for the primary calcium phosphate, Ca(H~2~PO~4~