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Rapid determination of adenoviral vector titers by quantitative real-time PCR

✍ Scribed by Libin Ma; Hans A.R. Bluyssen; Marc De Raeymaeker; Veerle Laurysens; Niels van der Beek; Heidi Pavliska; Anton-Jan van Zonneveld; Peter Tomme; Helmuth H.G. van Es

Book ID: 114139630
Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 549 KB
Volume: 93
Category: Article
ISSN: 0166-0934
DOI: 10.1016/s0166-0934(01)00257-9

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## Abstract Rapid diagnosis of human adenovirus (HAdV) infections was achieved by PCR in the recent years. However, conventional PCR has the risk of carry‐over contamination due to open handling with its products, and results are only qualitative. Therefore, a quantitative “real‐time” PCR with cons