Rapid determination of 4-aminobutyric acid and L-glutamic acid in biological decarboxylation process by capillary electrophoresis-mass spectrometry

✍ Scribed by Sahori Takeda; Naoko Yamano; Norioki Kawasaki; Hisanori Ando; Atsuyoshi Nakayama

• Publisher: John Wiley and Sons
• Year: 2012
• Tongue: English
• Weight: 179 KB
• Volume: 35
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201100776

✦ Synopsis

4-Aminobutylic acid (GABA) is a monomer of plastic polyamide 4. Bio-based polyamide 4 can be produced by using GABA obtained from biomass. The production of L-glutamic acid (Glu) from biomass has been established. GABA is produced by decarboxylation of Glu in biological process. High-performance liquid chromatography (HPLC) with derivatization is generally used to determine the concentration of GABA and Glu in reacted solution samples for the efficient production of GABA. In this study, we have investigated the rapid determination of GABA and Glu by capillary electrophoresis-mass spectrometry (CE-MS) without derivatization. The determination was achieved with the use of a shortened capillary, a new internal standard for GABA, and optimization of sheath liquid composition. Determined concentrations of GABA and Glu by CE-MS were compared with those by pre-column derivatization HPLC with phenylisothiocyanate. The determined values by CE-MS were close to those by HPLC with pre-column derivatization. These results suggest that the determination of GABA and Glu in reacted solution is rapid and simplified by the use of CE-MS.