Rapid Detection of Translation-Terminating Mutations at the Adenomatous Polyposis Coli (APC) Gene by Direct Protein Truncation Test

Simple, rapid, and accurate determinatio

Simple, rapid, and accurate determination of deletion mutations by automated dna sequencing of heteroduplex fragments of the adenomatous polyposis coli (APC) gene generated by PCR amplification

✍ Kazuo Tamura; Yoshihiro Yamamoto; Yoshifumi Saeki; Jun-ichi Furuyama; Joji Utsun 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 628 KB

Germline mutations in patients with familial adenomatous polyposis were analyzed by polymerase chain reaction (PCR) amplification of the adenomatous polyposis coli gene. PCR products from heterozygous patients for deletions of this gene formed four distinct bands on polyacrylamide gel electrophoresi