Rapid detection of serum HCV RNA by combining reverse transcription and PCR without RNA extraction
β Scribed by Jeong Su Jang; Kong-Joo Lee
- Book ID
- 112963083
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 549 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0253-6269
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Eleven children with acute encephalopathy associated with an influenza virus infection were treated during the 1997-1998 influenza season. Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect the viral genome in peripheral blood and cerebrospinal fluid (CSF) samples. The
Serum samples from 103 blood donors or patients with slightly increased serum levels of liver enzymes were tested for antibodies to hepatitis C virus (anti-HCV) using second generation tests and for HCV RNA by the polymerase chain reaction (PCR). PCR was in a nested configuration, using primer pairs