Rapid detection of polyhydroxyalkaloid mono- and diglycosides in crude plant extracts by direct quadrupole ion trap mass spectrometry

✍ Scribed by Geoffrey C. Kite; Elaine A. Porter; Martin J. Egan; Monique S. J. Simmonds

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 83 KB
• Volume: 10
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(199909/10)10:5<259::aid-pca463>3.0.co;2-8

✦ Synopsis

Direct MS analysis, utilizing first-order MS and subsequent MS 2 and MS 3 product ion analyses, is shown to provide a rapid means of characterizing polyhydroxyalkaloid glycosides and aglycones in aqueous methanol plant extracts that have been crudely purified on ion exchange resin. Analysis of species known to synthesize polyhydroxyalkaloids resulted in the discovery of the first diglycosides of these compounds. These were detected in Omphalea diandra and Syzygium oleosum. A monoglycoside was also detected as a minor component in Castanospermum australe.