Rapid detection of cytomegalovirus by tissue culture, centrifugation, and immunofluorescence with a monoclonal antibody to an early nuclear antigen
✍ Scribed by Geoffrey M. Thiele; Molly S. Bicak; Anita Young; Joyce Kinsey; Roberta J. White; David T. Purtilo
- Book ID
- 119114421
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 705 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0166-0934
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A commercially available monoclonal antibody against the 72000 Dalton early nuclear protein (EA) of cytomegalovirus (CMV) strain AD169 was used in an indirect immunofluorescence staining procedure (IF) for rapid detection of CMV-infected cells in tissue cultures inoculated with clinical specimens (2
## Abstract Skin fibroblasts exposed to cytosine arabinoside (Ara C) were infected with either cytomegalovirus (CMV) or herpes simplex virus (HSV) type 1 and 2. Herpesvirus‐determined early antigens (HV‐EA), detected by anti‐complement immunofluorescence (ACIF), occurred primarily in the nuclei, an