Rapid detection of all HLA-B*27 alleles (B*2701–B*2725) by group-specific polymerase chain reaction

✍ Scribed by E. Zino; S. Di Terlizzi; C. Carugo; L. Baggi; A. Galli; C. Bonini; S. Rossini; B. Mazzi; K. Fleischhauer

• Book ID: 110891969
• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 259 KB
• Volume: 63
• Category: Article
• ISSN: 0001-2815
• DOI: 10.1111/j.1399-0039.2004.00158.x

✦ Synopsis

Abstract: Human leucocyte antigen‐B*27 is strongly associated with a number of rheumatic diseases, including ankylosing spondylitis and reactive arthritis. Targeted detection of the B*27 group by molecular methods is hampered by the extreme heterogeneity of the serological B*27 group. Here, we describe a simple, rapid sequence‐specific primer‐based method for detection of all 28 B*27 alleles defined to date. The method involves an initial screening with two sequence‐specific polymerase chain reactions (PCRs), which has to be followed by two additional PCR amplifications in samples carrying a few rare subtypes of B*27, B*4202 or B*7301. The described protocol should be useful for laboratories involved in diagnostics and research of rheumatoid diseases.