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Rapid detection and quantitation of hepatitis B virus DNA by real-time PCR using a new fluorescent (FRET) detection system

✍ Scribed by Sani Hussein Aliyu; Muktar Hassan Aliyu; Hamisu M Salihu; Surendra Parmar; Hamid Jalal; Martin David Curran


Book ID
116630572
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
88 KB
Volume
30
Category
Article
ISSN
1386-6532

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Real-time PCR for detection and quantita
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## Abstract A sensitive and reproducible real‐time PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an β€œin‐house” qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B pati