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Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis

✍ Scribed by H. M. Pham; S. Konnai; T. Usui; K. S. Chang; S. Murata; M. Mase; K. Ohashi; M. Onuma


Publisher
Springer Vienna
Year
2005
Tongue
English
Weight
203 KB
Volume
150
Category
Article
ISSN
1432-8798

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## Abstract We developed a real‐time PCR protocol to detect orthopoxviruses (OPVs) from different clinical specimens and to separate variola virus from other OPVs. In our protocol, we used automated nucleic acid extraction system together with real‐time PCR to create a simple, safe and fast procedu