𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Rapid density gradient centrifugation using Short Column Techniques

✍ Scribed by Owen M. Griffith

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
417 KB
Volume
90
Category
Article
ISSN
0003-2697

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✦ Synopsis

The Short Column Method consists of partially filling the tubes. In the case of rate zonal runs, sucrose gradients, for example, were used to fill the tube to one-half the volume and the sample was carefully layered on top.

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Coagulation of polystyrene latices was followed by using a combination of centrifugation in density gradients and lightscattering densitometry. Second-order rate constants \(\left(k_{11}\right)\) were obtained under various conditions. At \(25^{\circ}\) and \(45^{\circ} \mathrm{C}\) under 0.5 \(\mat