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Rapid combined genotyping of factor V, prothrombin and methylenetetrahydrofolate reductase single nucleotide polymorphisms using minor groove binding DNA oligonucleotides (MGB probes) and real-time polymerase chain reaction

✍ Scribed by Louis, Magali; Dekairelle, Anne France; Gala, Jean-Luc

Book ID: 120310676
Publisher: Walter de Gruyter GmbH & Co. KG
Year: 2004
Tongue: English
Weight: 181 KB
Volume: 42
Category: Article
ISSN: 1434-6621
DOI: 10.1515/CCLM.2004.255

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## Communicated by Mireille Claustres Novel fluorescent oligonucleotides that contain a 3¢ minor groove binding group (MGB) hybridize to single-stranded targets with increased sequence-specificity compared to ordinary DNA probes. This reduces non-specific probe hybridization and results in low bac