Rapid Cleavage of Oligodeoxyribonucleotides from cis-Diol-Bearing Universal Polymer Support

✍ Scribed by Pradeep Kumar; Kailash C. Gupta

• Book ID: 102253528
• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: German
• Weight: 93 KB
• Volume: 86
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.200390019

✦ Synopsis

Abstract

Two sets of conditions have been proposed for the cleavage of oligodeoxyribonucleotides from cis‐diol‐bearing polymer supports and their complete deprotection. The first condition involves the use of spermine in the presence of Zn^2+^ ions in aqueous NH~3~ at 60° for 1 h, while the second one employs the use of 0.5M LiOH in conjunction with 3.5M Et~3~N/MeOH at 75° for 40–60 min, or under microwaves for 5–8 min, depending on the protection employed for nucleic bases.