Rapid chemiluminometric determination of gabapentin in pharmaceutical formulations exploiting pulsed-flow analysis

✍ Scribed by Matías Manera; Manuel Miró; Marta F. T. Ribeiro; José Manuel Estela; Víctor Cerdà; João L. M. Santos; José L. F. C. Lima

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 201 KB
• Volume: 24
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1055

✦ Synopsis

Abstract

In this study, a straightforward and automated pulsed flow‐based procedure was developed for the chemiluminometric determination of gabapentin [1‐(aminomethyl)cyclo‐hexaneacetic acid], a new generation antiepileptic drug, in different formulated dosage forms. The software‐controlled time‐based injection method capitalizes on the decrease of the background chemiluminescence (CL) readout of the luminol–hypochlorite reaction in the presence of gabapentin. In short, gabapentin works as a hypochlorite scavenger. The analytical procedure was implemented in a multi‐pumping flow network furnished with a suite of microdispensing solenoid‐actuated pumps. The diaphragm‐type micropumps might be configured to operate as fluid propellers, commutation units and metering injectors. A dynamic linear working range for gabapentin concentrations in the range 60–350 µmol/L was obtained, with an estimated detection limit of 40 µmol/L. The flow analyser handles about 41 injections/h and yields precise results (RSD < 2%). The miniaturized flow analyser thus has potential to be exploited for in‐line monitoring of drug manufacturing within the quality assurance framework of modern pharmaceutical companies. Copyright © 2008 John Wiley & Sons, Ltd.