Rapid aqueous photo-polymerization route to polymer and polymer-composite hydrogel 3D inverted colloidal crystal scaffolds
✍ Scribed by Yuanfang Liu; Shaopeng Wang; Justin Krouse; Nicholas A. Kotov; Mohammad Eghtedari; Gracie Vargas; Massoud Motamedi
- Book ID
- 102295022
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 429 KB
- Volume
- 83A
- Category
- Article
- ISSN
- 1549-3296
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Successful regeneration of biological tissues in vitro requires the utilization of three‐dimensional (3D) scaffolds that provide a near natural microenvironment for progenitor cells to grow, interact, replicate, and differentiate to form target tissues. In this work, a rapid aqueous photo‐polymerization route was developed toward the fabrication of a variety of polymer hydrogel 3D inverted colloidal crystal (ICC) scaffolds having different physical and chemical properties. To demonstrate the versatility of this technique, a variety of polymer hydrogel ICC scaffolds were prepared, including (1) polyacrylamide (pAAM) scaffolds, (2) poly(2‐hydroxyethyl methacrylate) (pHEMA) scaffolds, (3) poly(2‐hydroxyethyl acrylate) (pHEA) scaffolds, and composite scaffolds including (4) pAAM‐pHEMA scaffolds, (5) pHEMA‐pMAETAC [poly(2‐methacryloyloxy) trimethyl ammonium] scaffolds, and (6) pHEA‐pMEATAC scaffolds. Templates for scaffolds incorporated both uniform sized (104 μm diameter) and nonuniform sized (100 ± 20 μm diameter) closely packed noncrosslinked poly(methyl methacrylate) beads. Human bone marrow stromal HS‐5 cells were cultured on the six different types of scaffolds to demonstrate biocompatibility. Experimental results show that cells can remain viable in these scaffolds for at least 5 weeks. Of the six scaffolds, maximal cell adhesion and proliferation are obtained on the positively charged composite hydrogel pHEMA‐pMEATAC and pHEA‐pMAETAC scaffolds. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007