Rapid and specific cyclosporine assay for the du pont aca® discrete clinical analyzer performed directly on whole blood
✍ Scribed by Joel B. Hansen; H. Phillip Lau; Christopher J. Janes; Warren K. Miller; Donald A. Wiebe
- Book ID
- 102877639
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 536 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
A rapid, sensitive, and specific enzyme immunometric assay for cyclosporine in whole blood has been developed for the aca@ analyzer, using a monoclonal antibody from Sandoz, Ltd. Between-run CVs ranged from 6.5 to 7.6% for samples containing between 60 and 400 ngImL cyclosporine. Sensitivity was better than 25 ngImL in the assay, which has an effective upper range of greater than 600 ngImL. Two correlation studies compared cyclosporine values from the Du Pont method to those determined by HPLC procedures in three hospital laboratories. The results from a total of 120 whole blood samples with CsA between 20 and 800 ngImL showed excellent correlation between the methodologies. HPLC and Du Pont CsA values from 10 day serial studies also correlated well for samples from a kidney, kidney-pancreas, heart, and two liver transplant patients. We conclude that the Du Pont CSA assay provides accurate and reproducible results in a convenient format in less than 30 minutes.