Rapid and simple synchronization of mammalian suspension cell cultures involving minimal damage to DNA

The application of rapid kinetic techniq

The application of rapid kinetic techniques to the transport of thymidine and 3-O-methylglucose into mammalian cells in suspension culture

✍ Robert M. Wohlhueter; Richard Marz; Jon C. Graff; Peter G. W. Plagemann 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 636 KB

## Abstract Techniques are described by which the transport of nutrients into mammalian cells in suspension can be measured at intervals of 1.5 seconds. By application of these techniques, the existence of a saturable (K~m~ = 85 μM), non‐concentrative, transport system for thymidine was demonstrate