Rapid and simple method for the identification of apolipoprotein E isomorphic phenotypes from whole serum
✍ Scribed by Dr. Martin Kohlmeier; Hans-Joachim Drossel; Pranav Sinha; Eckart Köttgen
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 431 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
For the identification of apolipoprotein E isomorphic phenotypes,fresh or thawed serum was analyzed without prior delipidation or other pretreatment. Using 5 O/ o polyacrylamide gels with a 40 m m interelectrode distance, the isoforms were separated by isoelectric focusing in immobilized pH gradients ranging from pH 5 to 6.5, and transferred onto polyvinylidene difluoride membranes by contact blotting for 1 h. The apolipoprotein E isoforms were identified following immunostaining. The electrophoresis required less than 2 h and the entire procedure could be completed within 6 h.