✦ LIBER ✦
Rapid and sensitive typing of forensic stains by PCR amplification of polymorphic simple repeat sequences in case work
✍ Scribed by Lutz Roewer; Jörg T. Epplen
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 971 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0379-0738
No coin nor oath required. For personal study only.
✦ Synopsis
After failure of conventional typing and multilocus DNA fingerprinting methods to compare a minute vaginal swab stain with blood from a murder victim and a suspect, we used enzymatic DNA amplification (polymerase chain reaction, PCR) to discriminate the DNAs by typing of simple sequence lengths polymorphisms. A mixed dinucleotide locus in the HLA-DRB gene region and three novel tetranucleotide polymorphisms located autosomally as well as on the human Y chromosome were used to exclude a jailed subject from a case of murder.