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Rapid and sensitive quantitation of the antiproliferative agent mitoguazone in small volumes of plasma by high-performance liquid chromatography with ultraviolet detection

โœ Scribed by Ching-Ling Cheng; E-Gin Lin; Chen-Hsi Chou


Book ID
108497501
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
178 KB
Volume
793
Category
Article
ISSN
1873-376X

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Rapid Quantitative Determination of Sial
โœ K.R. Anumula ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 563 KB

Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co