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Rapid and sensitive DNA target detection using enzyme amplified electrochemical detection based on microchip

✍ Scribed by Wei Cao; Mingming Su; Shusheng Zhang


Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
258 KB
Volume
31
Category
Article
ISSN
0173-0835

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A novel strategy is described for highly sensitive DNA detection and point mutation identification based on the combination of reverse molecular beacon with DNA ligase. A 5'-phosphoryl and 3'-ferrocene terminated DNA sequence is used as detection probe, which may be ligated to capture DNA immobilize