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Rapid and sensitive detection of D-hydantoinase producing microorganisms by using microtiter plate assay

✍ Scribed by Hungchien Roger Chien; Wen-Hwei Hsu


Book ID
104645947
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
288 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis


D-Hydantoinase activity of microorganisms was detected by a microtiter plate assay using dihydrouracil as substrate in 24 h. The detection gave a clear result compared to the spot-test in which the colour development of one colony interfered with colour development by adjacent colonies. The sensitivity of the detection was also higher than that of the spot-test.


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