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Rapid and reliable protein extraction from yeast

✍ Scribed by Vitaly V. Kushnirov

Book ID
101321468
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
115 KB
Volume
16
Category
Article
ISSN
0749-503X

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A simple and reliable method for the efficient inactivation of metabolism and for quantitative metabolite extraction from yeast cells is presented. It is based on the use of a boiling solution made of 75% ethanol (volume/final volume) buffered with 70 mM-Hepes (final concentration), pH 7.5, to guara