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Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction

✍ Scribed by R. D'Ovidio; O. A. Tanzarella; E. Porceddu

Publisher
Springer
Year
1990
Tongue
English
Weight
942 KB
Volume
15
Category
Article
ISSN
0167-4412

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✦ Synopsis

The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.

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