Rapid analysis of protein interactions: On-chip micropurification of recombinant protein expressed in Esherichia coli

Genetic analysis of G protein-coupled re

Genetic analysis of G protein-coupled receptor expression in Escherichia coli: Inhibitory role of DnaJ on the membrane integration of the human central cannabinoid receptor

✍ Georgios Skretas; George Georgiou 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 644 KB

## Abstract The overexpression of G protein‐coupled receptors (GPCRs) and of many other heterologous membrane proteins in simple microbial hosts, such as the bacterium __Escherichia coli__, often results in protein mistargeting, aggregation into inclusion bodies or cytoplasmic degradation. Furtherm