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Rapid analysis of 3′-ends of DNA fragments by terminal 32P-labeling

✍ Scribed by Alberto Bernardi; Umberto Bertazzoni


Book ID
107711628
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
764 KB
Volume
61
Category
Article
ISSN
0003-2697

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Cleavage of 3'-end-labeled DNA in hot aqueous solutions of different amines is comparatively examined for overall rate of DNA scission as well as for potential differences in the preference of the various amines for cleavage at the different bases. Under comparable conditions (0.5 M amine, 0.3 M NaC