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Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E

✍ Scribed by Chariklia Economou; Keqin Chen; Frances H. Arnold


Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
540 KB
Volume
39
Category
Article
ISSN
0006-3592

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πŸ“œ SIMILAR VOLUMES


Solvent effects on the catalytic activit
✍ Teresa CorrΓͺa de Sampaio; Rui B. Melo; Teresa F. Moura; Stefan Michel; Susana Ba πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 907 KB

We studied a model transesterification reaction catalyzed by subtilisin Carlsberg suspended in toluene, n-hexane, diisopropyl ether, and mixtures of these solvents. To account for solvent effects due to differences in water partitioning between the enzyme and the bulk solvents, we measured water sor