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Random insertion of Tn4560inStreptomyces lividans and Streptomyces avermitilis

✍ Scribed by Susanne Irnich; John Cullum

Book ID
104634373
Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
834 KB
Volume
15
Category
Article
ISSN
0141-5492

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✦ Synopsis

Pulsed-field gel electrophoresis (PFGE) was used to show that insertions of transposon Tn4560 in Streptomyces lividans 66 and the avermectin-producer S. avermitilis are randomly distributed. DNA from the latter strain suffered degradation during electrophoresis that could be avoided by modification of the buffer. Inverse PCR primers were developed for Tn4560.

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