Raman spectroscopic study of uridine nucleotides: structural information and conformational features

✍ Scribed by P. Carmona; A. Rodríguez-Casado; M. Molina; R. Escobar

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 98 KB
• Volume: 30
• Category: Article
• ISSN: 0377-0486
• DOI: 10.1002/(sici)1097-4555(199908)30:8<631::aid-jrs404>3.0.co;2-n

✦ Synopsis

Raman spectra of the sodium salts of uridine and deoxyuridine monophosphoric acid, Na 2 (3 -UMP)•4H 2 O, Na 2 (5-UMP)•7H 2 O and Na 2 (5 -dUMP)•5H 2 O, and the cadmium salt, [Cd(5 -UMP)(H 2 O) 2 ]•2H 2 O, were examined. Several spectral differences were observed owing to nucleoside conformational differences in the uridine residue. The spectroscopic characterization of the nucleoside structures was made on the basis of previous x-ray crystal studies of these compounds. Although the crystal structures of Ca(3 -UMP)•2H 2 O, Sr(3 -UMP)•4H 2 O and Ba(3 -UMP)•4H 2 O also reported here are as yet unknown, their nucleoside structures were inferred from the position of the phosphoester stretching band. These results enabled us to consider some novel Raman band markers as diagnostic of the C(3 )-endo and C(2 )-endo uridine conformations.