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Raman spectroscopic study of the avidin—biotin complex

✍ Scribed by C. Fagnano; G. Fini; A. Torreggiani

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
474 KB
Volume
26
Category
Article
ISSN
0377-0486

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## SYNOPSIS FT Raman spectra of avidin, streptavidin, and of the two anhydrous biotin complexes were obtained by excitation in near infrared ( NIR) . As far as avidin and the avidin-biotin complex are concerned, the excitation in NIR did not change the main features of the spectra compared with th

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Protein multilayers were prepared on silica surfaces by up to 20 alternating incubations of a biotin-protein conjugate and polymerized streptavidin. Spectroscopic ellipsometry (350-750 nm) was used to investigate the physical thickness and the dispersion of the protein layer after each incubation st

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✍ A. Torreggiani; G. Fini 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 295 KB 👁 2 views

Raman spectra of anhydrous complexes of streptavidin (Strep) with biotin (Bio) and some Bio analogues [Biotin methyl ester (MEBio), desthiobiotin (DEBio), 2-iminobiotin (IMBio), and diaminobiotin (DABio)] were recorded. The vibrational results indicate that the interaction with some of these ligands