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Raman difference studies of protein–nucleic acid interactions

✍ Scribed by Hua Deng; Robert Callender

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
105 KB
Volume
30
Category
Article
ISSN
0377-0486

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✦ Synopsis

Raman difference spectroscopy combined with isotope editing procedures were employed to obtain the Raman spectra of phosphates, nucleotides, nucleic acids and their fragments when bound to several proteins. The vibrational spectra were analyzed using a variety of empirical and ab initio methods to relate the vibrational wavenumbers to hydrogen bonding strengths, bond orders, bond lengths and geometry and to establish how the proteins bring about these interactions. Some recent results obtained using these methods are reviewed.

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