20500 h o , Finland (1.H.I SH-SY5Y human neuroblastoma cells can be induced to differentiate into a neuronal phenotype by treatment with 12-O-tetradecanoylphorbol-13-acctatc UPA). In other cell systems, TPA treatment frequently leads to down-regulation of protein kinase C (PKC). However, we now rep
Raf Kinase Inhibitor Protein Enhances Neuronal Differentiation in Human SH-SY5Y Cells
✍ Scribed by Hellmann, Julian; Rommelspacher, Hans; Mühlbauer, Eckhard; Wernicke, Catrin
- Book ID
- 126670898
- Publisher
- S. Karger AG
- Year
- 2010
- Tongue
- English
- Weight
- 731 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0378-5866
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## Abstract Neuroblastomas are pediatric tumors originating from immature neuroblasts in the developing peripheral nervous system. Differentiation therapies could help lowering the high mortality due to rapid tumor progression to advanced stages. Oleic acid has been demonstrated to promote neuronal
SH-SYSY cells differentiate into neuronal-like cells and express marker proteins like growth-associated protein (GAP-43) and neuropeptide tyrosine when treated with a low concentration (16 nM) of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of growth factors or serum.