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Radiotracer investigation of a quantitative thin-layer chromatographic separation

✍ Scribed by R.A.G. Marshall; J.F. Lockyer

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
605 KB
Volume
166
Category
Article
ISSN
1873-3778

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✦ Synopsis

Radioactive labelling has been shown to be a powerful technique for investigating quantitative thin-layer chromatography. The dispensing of small volumes by several appliances has been shown to be precise but biased to the extent of several percent by "creep back" of the dispensed solution on the needle of the syringe. Losses in the thin-layer chromatographic separation and recovery of phthalimide once the solution had been dispensed totalled approximately 3 %. These losses were attributed to a residue at the site of application to the plate, underestimating the size of the developed spot and the failure of a fraction of the phthalimide to elute.

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